补肾壮骨胶囊对去卵巢大鼠骨质疏松症骨密度和相关细胞因子的影响

目的：研究补肾壮骨胶囊对去卵巢大鼠骨质疏松症的骨密度和细胞因子等的影响,探索该药对绝经后骨质疏松症的防治功效及作用机理。方法：将大鼠随机分为6组：假手术组、卵巢切除组、切除卵巢＋牡蛎碳酸钙组、切除卵巢＋补肾壮骨胶囊低剂量组、切除卵巢＋补肾壮骨胶囊中剂量组、切除卵巢＋补肾壮骨胶囊高剂量组,观察骨密度、血清E2和IL-6、子宫指数和股骨指数等指标,了解其对去卵巢大鼠骨骼的影响。结果：1）骨密度的变化：去卵巢16周后,卵巢切除组大鼠右股骨骨密度和骨矿含量均明显低于假手术对照组,差异有显著性（P〈0.01）,表明造模成功。与卵巢切除组比较,补肾壮骨胶囊中剂量组和高剂量组可明显提高模型大鼠股骨BMC含量（P〈0.05）,高剂量组可明显提高模型大鼠股骨BMD的含量（P〈0.05）。2）血清E2和IL-6的变化：去卵巢16周后,卵巢切除组大鼠血清E2含量显著低于假手术对照组,差异有显著性（P〈0.05）。中药中剂量组及高剂量组E2含量明显高于模型组,差异有显著性（P〈0.05）。3）子宫指数和股骨指数：与假手术组比较,卵巢切除组子宫指数和股骨指数明显降低,具有统计学意义（P〈0.05）,提示造模成功。与卵巢切除组比较,高剂量组子宫指数明显增加（P〈0.05）;高、中、低三个剂量组股骨指数均明显增加（P〈0.05）。结论：补肾壮骨胶囊能增加去卵巢大鼠子宫湿重,但对干重无明显影响,可以显著提高去卵巢大鼠离体骨骨密度及改善其生物力学性能,提高去卵巢大鼠血清E2和IL-6含量,具有类性激素样作用。     

Effects of the Drug （BSZGC）-containing Serum on Proliferation of Rat＇s Osteoclasts and TRACP Activity in vitro

Objective： To observe effects of the drug-containing serum ofBu Shen Zhuang Gu Capsule （BSZGC 补肾壮骨胶囊 Capsule for Tonifying the Kidney to Strengthen the Bones） on proliferation of the rat＇s osteoclasts and tartrate-resistant acid phosphatase （TRACP） activity in vitro so as to delve into the mechanisms of its preventive and therapeutic actions on osteoporosis. Methods： Forty female Sprague-Dawley rats aged three months were randomly divided into high dosage BSZGC group, medium dosage BSZGC group, low dosage BSZGC group, and the control group. BSZGC was orally administered into the rats of high, medium, and low dosage groups at different dosages for 12 days, and isometric normal saline was orally administered to the rats of the control group. The drug-containing serum and control serum were prepared. Osteoclasts isolated mechanically from the femur and tibia of Sprague-Dawley rats aged one week were cultured with medium added with different drug-containing sera and control serum. The growth of osteoclasts was observed under an inverted phase-contrast microscope, and optic density （OD） value of osteoclasts was determined by MTT colorimetric assay. TRACP activity was measured by the diazol method. Results： OD value of osteoclasts in the high dosage drug-containing serum group, medium dosage drug-containing serum group, and low dosage drug-containing serum group was significantly lower than that in the control serum group （P〈0.05） with a dose-effect correlation. TRACP activity in high dosage drug-containing serum group, medium dosage drug-containing serum group, low dosage drug-containing serum group was significantly lower than that of the control serum group （P〈0.01）, and no significant differences in TRACP activity were not found among the different dosages drug-containing serum groups. Conclusions： BSZGC can inhibit the proliferation of the osteoclasts and reduce TRACP activity, which may be one of the mechanisms of its preventive and theraoeutic actions on osteooorosis     

沙棘医疗保健作用

沙棘具有抗癌抑瘤、增加免疫力的功能,能祛痰利肺,协调肝、胃、脾、肾和心脏功能的平衡,能活血化瘀、消炎生肌,也能防辐射、抗衰老。     

原发性肾小球疾病中医证型与舌形指数关系的探讨

目的：探讨原发性肾小球疾病中医证型与舌形指数之间的关系。方法：将116例病例按中医辨证本证分为气虚、阳虚、阴虚、气阴两虚4型，标证分为水湿、湿热、湿浊和瘀血4型，观察舌形；利用专用舌象信息处理仪器，采集舌形数据，经计算机处理输出舌形指数。通过肉眼观察和计算机对所采集舌形的定量分析（舌形指数），探讨各中医证型舌形指数间的差异。结果：各证型之间的舌形指数差异均无统计学意义（P〉0．05）。结论：胖大齿痕舌与气虚水湿证有关，舌形指数对中医辨证分型有一定的指导意义。     

心复康Ⅲ号的急性毒性及药效学研究

心复康Ⅲ号对小鼠的急性毒性用简化机率法计算IpLD_(50)为79.74±4.14g/kg,PoLD_(50)为824.3±119.45 g/kg。心复康Ⅲ号对动物常压耐缺氧、异丙肾上腺素引起的耗氧量增加,亚硝酸钠、氰化钾中毒、结扎双侧颈总动脉引起的脑缺氧以及恒速静脉滴注戊巴比妥钠引起的心衰均有明显地保护作用。提示心复康Ⅲ号在常用剂量时基本无毒性并对缺血性心脏病有一定治疗作用。     

中医药调节免疫机能减少肾病综合征复发

根据肾病综合征的发生发展与机体免疫功能失调密切相关，总结４８例治疗经验，认为和解枢机，升发少阳可调节免疫状态；增强稳定，健脾补肾护卫可提高免疫功能。以汤药治疗获得很好的疗效，其中显效１４例，好转３０例，无效４例，有效率达９１．６％，且大部分病人胳药后血清ＩｇＧ明显升高，尿蛋白减少。结果表明和解、扶正中药有调节免疫机能，减少复发的作用。     

成骨诱导后人脐血间充质干细胞移植对兔骨折愈合的实验研究

目的探讨成骨诱导后的人脐血间充质干细胞(HUCBMSCs)移植对兔模型骨折愈合的影响。方法严格无菌条件下采集健康产妇志愿捐献的脐带血,采用密度梯度离心和贴壁培养法分离培养HUCBMSCs。取第3代细胞应用条件培养基进行成骨诱导2周;4~5月龄健康新西兰大白兔48只,体质量2.0~2.5 kg,雌雄不限,用线锯制作右胫骨横断骨折模型。将其分为A、B两组,每组又分为4个时点组,每小组6只。A组骨折断端注射成骨诱导后的HUCBMSCs;B组注射生理盐水。细胞移植后2、4、6、8周进行影像学、骨密度检测、组织学观察,观察评估骨折愈合情况。结果 2~8周X线片示:A组的骨折愈合速度要明显快于B组;组织学观察:A组新生骨小梁及类骨质明显多于B组;细胞移植后各时间点A组影像学结合组织学评分显著高于B组,两组比较差异有统计学意义(P<0.05);骨密度检测:细胞移植后第2、4、6周A组骨密度高于B组,两组比较差异有统计学意义(P<0.05),而在第8周A组骨密度低于B组,两组比较差异有统计学意义(P<0.05)。结论成骨诱导后的HUCBMSCs移植能明显促进兔骨折愈合。     

银芩合剂毒性试验研究

对银芩合剂进行的毒性试验研究表明,急性毒性试验无动力死亡,小鼠最大耐受量为８０ｇ．ｋｇ＾－１;大鼠完整皮肤及破损皮肤吸收毒性试验未见血象、血糖及肝肾功能改变,体重有增加;皮肤、肝及肾脏病理组织学检查无异常;对大鼠呼吸道粘膜及家兔眼无刺激性。不引起豚鼠皮肤过敏反应。